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Soni, Hardik
- Development and Validation of Analytical Method for Estimation of Calcium in Herbomineral Formulation by Atomic Absorption Spectrophotometry
Abstract Views :88 |
PDF Views:69
Authors
Affiliations
1 Faculty of Pharmacy, Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia, Vadodara, Gujarat, IN
2 Vasu Health Care, 967/4, G.I.D.C road, G.I, D.C, Makarpura, Vadodara, Gujarat, IN
1 Faculty of Pharmacy, Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia, Vadodara, Gujarat, IN
2 Vasu Health Care, 967/4, G.I.D.C road, G.I, D.C, Makarpura, Vadodara, Gujarat, IN
Source
Journal of Natural Remedies, Vol 22, No 4 (2022), Pagination: 597-606Abstract
Calcium determination is normally done by conventional titrimetric methods (complexometric titration). In this study, a simple, precise, accurate, rapid and sensitive method has been developed for calcium (Ca) determination in Maxcal-C Tablet (herbo-mineral formulation) and its raw materials by Atomic Absorption Spectrophotometer (AAS). For complete digestion of the sample, closed vessel wet digestion technique was used with 69% HNO3 and 30% H2O2 (7:1) as digestive solvents. Analysis was performed on AAS (AA-6300) at λmax 422.7 nm. Calcium nitrate Ca(NO3)2 was used as a standard for Calcium and the linearity of the standard was excellent over a concentration range of 0.5 to 20 μgmL-1. The regression coefficient (r2) was 0.999. For intraday and interday precision % RSD was obtained between 0.48%-1.95% and 3.48%-5.01%, respectively which complied with the standard range for AAS. Recovery was achieved at 93.24%, 99.73%, 101.75% for 3 levels of spikes (80%, 100%, 120%) respectively, which complied with the standard range 75- 125% for AAS. However, the AAS method produced accurate and reproducible results and also overcame the colour and other ions interference, which are common problems with the titrimetric method. The results of both techniques (the AAS method and the titrimetric method) were compared, which showed good estimation by AAS.Keywords
AAS, Bhasma, Complexometric Titration, Maxcal-C, Wet DigestionReferences
- Garg M, Das S, Jaspreet S, Sukender K. AS estimation of Heavy Metals and Trace elements in Indian Herbal Cosmetics Preparation. Research Journal of Chemical Science. 2012; 2(3):46-51.
- Rasmussen R, Hedegaard R. Development and validation of an SPE HG-AAS method for determination of inorganic arsenic in sample of marine origine. Anal Bioanal Chem. 2012; 2825-2834. https://doi.org/10.1007/s00216-012-6006-7 DOI: https://doi.org/10.1007/s00216-012-6006-7
- Ayivor, Okine L, Nyarko B, Debrah S. The application of Westcott Formalism k0 NAA method to estimate short and medium lived elements in some Ghanaian herbal medicines complemented by AAS. Radiation Physics and Chemistry. 2012; 81:403-409. https://doi.org/10.1016/j. radphyschem.2011.12.034 DOI: https://doi.org/10.1016/j.radphyschem.2011.12.034
- Carneiro D, Pedrini G, Teraza M, Henrique F. Determination of Na, K, Ca, Mg in biodiesel samples by flame AAS using microemulsion as sample preparation. Microchemical. 2010; 96:180-185. https://doi.org/10.1016/j. microc.2010.03.005 DOI: https://doi.org/10.1016/j.microc.2010.03.005
- Njoku P, Ohia C. Spectrophotometric estimation studies of Mineral nutrient in Three Cocoyam Cultivators. Pakistan Journal of Nutrition. 2007; 6:616-619.https://doi. org/10.3923/pjn.2007.616.619 DOI: https://doi.org/10.3923/pjn.2007.616.619
- Hseu Z. Evaluating heavy metal contents in nine composts using four digestion methods. Bioresource Technology. 2004; 95:53-59. https://doi.org/10.1016/j.biortech.2004.02.008 DOI: https://doi.org/10.1016/j.biortech.2004.02.008
- Soylak M, Tuzen M, Narin I, Sari H. Comparison of Microwave, Dry and Wet Digestion Procedures for the Determination of Trace Metal Contents in Spice Samples Produced in Turkey. Journal of Food and Drug Analysis. 2004; 12(3):254-258. https://doi.org/10.38212/2224-6614.2634 DOI: https://doi.org/10.38212/2224-6614.2634
- Bastos L, Soarse M, Correia L. Validation of an Electrothemal Atomic Absorption Spectrometry Method for the Determination of Aluminum, Copper and Lead in grapes. Toxicology Department, Faculty of Pharmacy, University of Porto, Portugal.
- Alarcon M, Lopez M, Lopez M, Segado A. Magnesium and Calcium content in food drom SE Spain: influencing factors and estimation pf daily dietary products. The Science of the Total Environment. 2003; 312:47-58. https:// doi.org/10.1016/S0048-9697(03)00199-2 DOI: https://doi.org/10.1016/S0048-9697(03)00199-2
- Mocak J, Borosova D, Miskovic P. Validation and Quality Assurance of Arsenic determination in Urine by GFAAS after toluene extraction. Polish Journal of Environmental studies. 2002; 11:617-623.
- Barbas C, Marin A, Gonzalvez A. Development and validation of extraction methods for determination of zinc and arsenic speciation in soils using focused ultrasound application to heavy metals study in mud and soils. Analytica Chimica Acta. 2001; 442:305-318. https://doi.org/10.1016/ S0003-2670(01)01169-2 DOI: https://doi.org/10.1016/S0003-2670(01)01169-2
- Soylak M, Dogan M, Narin I, Elci L. Determination of trace metal ions by AAS in natural water samples after preconcentration of pyrocatechol violet complexes on an activated carbon column. Talanta. 2000; 52:1041-1046. https://doi. org/10.1016/S0039-9140(00)00468-9 DOI: https://doi.org/10.1016/S0039-9140(00)00468-9
- Pybus J, Feldman F, Bowers G. Measurement of total Calcium in serum by Atomic Absorption Spectrometry with use of a strontium Internal Reference. Journal of clinical chemistry. 1970; 6:338-1007. https://doi.org/10.1093/ clinchem/16.12.998 DOI: https://doi.org/10.1093/clinchem/16.12.998
- Thin C, Thomson P. Estimation of Calcium and Magnesium in serum and urine by Atomic Absorption Spectrometry. Journal of clinical pathology.1967; 20:280-282. https://doi. org/10.1136/jcp.20.3.280 DOI: https://doi.org/10.1136/jcp.20.3.280
- Anthony P. Atomic absorption spectrometric determination of calcium and other metallic elements in some animal protein source. Talanta. 2000; 52:749-754. https://doi. org/10.1016/S0039-9140(00)00368-4 16. Krolak E, Zdanowski B. Phosphrous and calcium in the mussels Sinanodonta woodiana and dreissena polymorpha in konin lakes. Archieves of Polish Fisheries. 2007; 15:287-294. DOI: https://doi.org/10.1016/S0039-9140(00)00368-4
- Pinheiro J, Diniz A, Vasconcellos M, Soido C. An improvement of calcium determination technique in shell of Molluscs. Brazilian archieves of biology and technology. 2009; 52:93-98. https://doi.org/10.1590/S1516- 89132009000100012 DOI: https://doi.org/10.1590/S1516-89132009000100012
- Indian Pharmacopoeia, Indian pharmacopoeial commission. Ghaziabad, 2010; 2:962.
- Anti-BPH Activity of Polyherbal Formulation on Testosterone Induced Benign Prostatic Hyperplasia in Rats
Abstract Views :93 |
PDF Views:73
Authors
Affiliations
1 Department of Pharmacology, L.M. College of Pharmacy, Ahmedabad - 380009, Gujarat, IN
2 Vasu Research Center (A Division of Vasu Healthcare Pvt. Ltd.), G.I.D.C., Makarpura, Vadodara - 390010, IN
3 Department of Pharmacology, Parul Institute of Medical Science and Research, Parul University, Limda, Waghodia, Vadodara - 391760, Gujarat, IN
4 Department of Pharmacology, Parul Institute of Pharmacy and Research, Parul University, Vadodara, Gujarat - 391760, IN
1 Department of Pharmacology, L.M. College of Pharmacy, Ahmedabad - 380009, Gujarat, IN
2 Vasu Research Center (A Division of Vasu Healthcare Pvt. Ltd.), G.I.D.C., Makarpura, Vadodara - 390010, IN
3 Department of Pharmacology, Parul Institute of Medical Science and Research, Parul University, Limda, Waghodia, Vadodara - 391760, Gujarat, IN
4 Department of Pharmacology, Parul Institute of Pharmacy and Research, Parul University, Vadodara, Gujarat - 391760, IN
Source
Journal of Natural Remedies, Vol 22, No 4 (2022), Pagination: 617-627Abstract
Benign prostatic hyperplasia (BPH) is an enlargement of the prostate gland caused by progressive hyperplasia, or abnormal growth of cells of the glandular epithelial and stromal cells. Globally, it has been documented that more than 80% of men by the age of 80 will suffer from BPH. Most men are hesitant to undergo surgical interventions for fear of losing potency and the perception of other adverse side effects. Effectto® is a polyherbal formulation designed by Vasu Research Center to be used for the treatment of BPH. It can also be indicated that it can be used for lower urinary tract symptoms and bladder outlet obstruction. The formulation is expected to provide significant BPH relief. The present study was planned to evaluate the effect of a polyherbal formulation on testosterone- and citral-induced Prostatic Hyperplasia. The activity of the polyherbal formulation for BPH was evaluated using testosterone depot injection as the inducing agent in a testosterone-induced model and citral-induced model for the atypical type of BPH in rats. Also, the acute toxicity study was done using OECD 423 guidelines to check the toxicity of the test compound. Data are expressed as mean ± SD and statistical significance was evaluated using one way ANOVA followed by Tukey’s multiple comparison tests. The polyherbal formulation was found to be safe at oral doses of 2000 mg/kg. Effectto® significantly decreased the weight of the prostate in the testosterone model as well as the citral model in rats. The effect on biochemical markers like serum PSA and TNF-α was also seen in both the cases. In particular serum PSA, the decrease was majorly significant in both the models when XIII compared. The formulation was able to reverse the effect of inducing agents on the prostate’s size.Keywords
Benign Prostatic Hyperplasia, Citral, Effectto®, Finasteride, LUTS, PSA, Testosterone.References
- Roehrborn C. Benign Prostatic Hyperplasia: An Overview. Rev Urol. 2005; 7(9):S3-S14.
- Alan C, Kirilmaz B, Koçoglu H, et al. Comparison of effects of alpha receptor blockers on endothelial functions and coagulation parameters in patients with benign prostatic hyperplasia. Prostatic diseases and male voiding dysfunction. Urol. 2011; 77:1439-43.https://doi.org/10.1016/j. urology.2010.10.019 DOI: https://doi.org/10.1016/j.urology.2010.10.019
- Abrams P. In support of pressure-flow studies for evaluating men with lower urinary tract symptoms. Urol. 1994; 44:4153- 155.https://doi.org/10.1016/S0090-4295(94)80119-3 DOI: https://doi.org/10.1016/S0090-4295(94)80119-3
- Patel N and Parsons K. Epidemiology and etiology of benign prostatic hyperplasia and bladder outlet obstruction Indian. J Urol. 2014; 30(2):170-176.https://doi.org/10.4103/0970- 1591.126900 DOI: https://doi.org/10.4103/0970-1591.126900
- McVary K. BPH: epidemiology and comorbidities. Am J Manag Care. 2006; 12(5):122-128.
- Miller J, Tarter T. Combination therapy with dutasteride and tamsulosin for the treatment of symptomatic enlarged prostate. [J]. Clin Interv Aging. 2009; 4:251-258.https://doi. org/10.2147/CIA.S4102 DOI: https://doi.org/10.2147/CIA.S4102
- Keong T. Diagnosis and treatment of benign prostate hyperplasia in Asia. Transl Androl Urol. 2015; 4(4):478-483.
- Gonzalez R, Kaplan S. First-line treatment for symptomatic benign prostatic hyperplasia: Is there a particular patient profile for a particular treatment? World J Urol. 2006; 24:360-6.https://doi.org/10.1007/s00345-006-0092-0 DOI: https://doi.org/10.1007/s00345-006-0092-0
- Henry B, Parwani A. Atypical adenomatous hyperplasia (adenosis) of the prostate: a case report with review of the literature. Diagn Pathol. 2008; 3(34).https://doi. org/10.1186/1746-1596-3-34 DOI: https://doi.org/10.1186/1746-1596-3-34
- Nahata A, Dixit V. Ameliorative effects of stinging nettle (Urtica dioica) on testosterone-induced prostatic hyperplasia in rats. Andrologia. 2012; 44(11):396–409. https://doi.org/10.1111/j.1439-0272.2011.01197.x DOI: https://doi.org/10.1111/j.1439-0272.2011.01197.x
- Ramani R, Panigrahy B, Sahu S, et al. Effect of menthe piperita in experimental Prostatic hyperplasia in Wistar albino rats. Int J Pharm Pharm Sci. 2014; 7(2).
- Barffour I, Boye A, Ernest A, et al. Histoprotective Effect of Essential oil from Citrus aurantifolia in Testosterone- Induced Benign Prostatic Hyperplasia. Rat Adv Uro. 2019. https://doi.org/10.1155/2019/3031609 DOI: https://doi.org/10.1155/2019/3031609
- Instruction Manual, the quantitative determination of Rat PSA concentration. https://www.genxbio.com/rat_elisa.html
- Instruction Manual, the quantitative determination of Rat TNF-α conc. https://www.raybiotech.com/ rat-tumor-necrosis-factor-alpha-elisa-kit-forserumplasma- and-cell-culture-supernatant/